KLONOWANIE DNA PDF

This characteristic is exploited in TOPO-cloning. The technique utilises the inherent biological activity of DNA topoisomerase I. The biological role of topoisomerase is to cleave and rejoin supercoiled DNA ends to facilitate replication. During replication , the enzyme digests DNA specifically at this sequence, unwinds the DNA and re-ligates it again at the 3' phosphate group of the thymidine base. The linear vector DNA already has the topoisomerase enzyme covalently attached to both of its strands' free 3' ends.

Author:Bajar Baran
Country:Libya
Language:English (Spanish)
Genre:Technology
Published (Last):11 May 2013
Pages:430
PDF File Size:3.75 Mb
ePub File Size:10.82 Mb
ISBN:590-3-65136-624-6
Downloads:19317
Price:Free* [*Free Regsitration Required]
Uploader:Voodoogul



Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Learn more. We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above. Cell Biology. Nucleic Acid Analysis. Drug Development. Human Identification. Molecular Diagnostics.

Protein Analysis. Applied Sciences. Infectious Diseases. Custom Manufacturing. Onsite Stocking. Format and QC. Automation Solutions. Student Resources.

Peer Reviewed Literature. Product Usage Information. Global Support. Local Sales Support. Your Cart. Current Items 0. Inkubacja przez 15 min. Czas inkubacji 2x 30 min. Zestaw do szybkiej ligacji. System na bazie membrany krzemionkowej. Transfekcja przebiega jak zwykle, tylko o wiele wydajniej!

Learn more Dismiss. Our website does not fully support your browser. How can we help you? Request a consult. Global Support Our customer and technical support experts are here to help! Explore Support Center. Add multiple products. Trawienie: Enzymy restrykcyjne. Alkaline Phosphatase, Calf Intestinal.

Americas Brazil. United States. Pacific Asia Australia. Korea, Republic of. Europe Austria. United Kingdom.

101 VERBAL PASSAGES EXAMKRACKERS PDF

TOPO cloning

This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong. From Wikimedia Commons, the free media repository. File information. Structured data. Captions English Add a one-line explanation of what this file represents.

BRADYGAMES USF4 PDF

Cloning and DNA Markers

Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Learn more. We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above. Twoje konto. Contact Customer Service.

POORNACHANDRA TEJASWI BOOKS PDF

File:Gene cloning PL.svg

.

Related Articles