CONCENTRATION MICELLAIRE CRITIQUE PDF

Lipopolysaccharides LPS are cell wall components of Gram-negative bacteria. These molecules behave as bacterial endotoxins and their release into the bloodstream is a determinant of the development of a wide range of pathologies. These amphipathic molecules can self-aggregate into supramolecular structures with different shapes and sizes. The formation of these structures occurs when the LPS concentration is higher than the apparent critical micelle concentration CMC a. Light scattering spectroscopy both static and dynamic was used to directly characterize the aggregation process of LPS from Escherichia coli serotype B6. Both structures were characterized in terms of molecular weight 5.

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Critical micelle concentration (CMC)

Critical micelle concentration CMC is defined as the concentration of detergents above which micelles are spontaneously formed. The CMC is important in biology because at concentrations above it the detergents form complexes with lipophilic proteins. Below this borderline, detergents merely partition into membranes without solubilising membrane proteins. Note: The molecular weights for some detergents are average values. Triton X, for example, can range between and over MW depending on synthesis. The exact molecular weight influences the CMC. Note: CMCs can vary with ionic strength and temperature.

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Critical micelle concentration

In colloidal and surface chemistry , the critical micelle concentration CMC is defined as the concentration of surfactants above which micelles form and all additional surfactants added to the system go to micelles. The CMC is an important characteristic of a surfactant. Before reaching the CMC, the surface tension changes strongly with the concentration of the surfactant. After reaching the CMC, the surface tension remains relatively constant or changes with a lower slope. The value of the CMC for a given dispersant in a given medium depends on temperature, pressure, and sometimes strongly on the presence and concentration of other surface active substances and electrolytes.

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Evaluation of Lipopolysaccharide Aggregation by Light Scattering Spectroscopy

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