CITRUS EXOCORTIS VIROID PDF

Contents - Previous - Next. The exocortis disease of citrus, caused by the citrus exocortis viroid CEV , was first reported and described by Fawcett and Klotz as a bark-shelling disorder of trifoliate orange rootstock. Benton et al. They recommended that budwood for propagation be taken only from older trees on trifoliate rootstock showing no scaling of the butt or trunk. The disease is present in almost all citrus-growing regions of the world.

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We'd like to understand how you use our websites in order to improve them. Register your interest. Surveys of Impatiens and Verbena species in local nurseries in Fredericton, Canada and Verbena species in New Delhi, India showed widespread infection of Citrus exocortis viroid CEVd in vegetatively-propagated and seed-grown plants.

To determine viroid seed transmission, samples of eight varieties of Impatiens and 11 varieties of Verbena were obtained from four commercial sources. All 19 samples collected contained viroid infection irrespective of variety. This is a preview of subscription content, log in to check access. Rent this article via DeepDyve. Bar-Joseph, M. Natural history of viroids—historical aspects. Hadidi, R. Flores, J. Semancik Eds.

Google Scholar. Bostan, H. An RT-PCR primer pair for the detection of Pospiviroid and its application in surveying ornamental plants for viroids.

Journal of Virological Methods , , — Duran-Vila, N. Citrus viroid. Fagoaga, C. Naturally occurring variants of Citrus exocortis viroid in vegetable crops. Plant Pathology , 45 , 45— A Citrus exocortis viroid variant from broad bean Vicia faba L. The Journal of General Virology , 76 , — Fawcett, H. Exocortis of trifoliate orange. Citrus Leaves , 28 , 8—9. Flores, R. Fauquet, D. Bishop, E. Carstens, M. Estes, S.

Lemon, et al. San Diego, CA: Academic. The sequence of a viroid from grapevine closely related to severe isolates of Citrus exocortis viroid. Nucleic Acids Research , 15 , — Garnsey, S. Mechanical transmission of exocortis virus with contaminated budding tools.

Plant Disease Reporter , 51 , — Ito, T. Simultaneous detection of six citrus viroids and Apple stem grooving virus from citrus plants by multiplex reverse transcription polymerase chain reaction. Mishra, M. Indian bunchy top disease of tomato plants is caused by a distinct strain of Citrus exocortis viroid. The Journal of General Virology , 72 , — Nie, X. Molecular cloning, secondary structure, and phylogeny of three pospiviroids from ornamental plants.

Canadian Journal of Plant Pathology , 27 , — Salibe, A. Reaction of types of citrus as scion and as rootstock to xyloporosis virus. Price Ed.

Semancik, J. Citrus exocortis viroid. Singh, R. Tomato chlorotic dwarf viroid in the ornamental plant Vinca minor and its transmission through tomato seed. European Journal of Plant Patholology , , — Detection of Citrus exocortis viroid , Iresine viroid , and Tomato chlorotic dwarf viroid in new ornamental host plants in India. Plant Disease , 90 , An alkaline solution simplifies nucleic acid preparation for RT-PCR and infectivity assays of viroids from crude sap and spotted membrane.

Verhoeven, J. Natural infections of tomato by Citrus exocortis viroid , Columnea latent viroid , Potato spindle tuber viroid and Tomato chlorotic dwarf viroid. European Journal of Plant Pathology , , — Download references. Correspondence to Rudra P. Reprints and Permissions. Citrus exocortis viroid transmission through commercially-distributed seeds of Impatiens and Verbena plants.

Eur J Plant Pathol , — Download citation. Received : 29 September Accepted : 29 January Published : 12 February Issue Date : August Search SpringerLink Search. Abstract Surveys of Impatiens and Verbena species in local nurseries in Fredericton, Canada and Verbena species in New Delhi, India showed widespread infection of Citrus exocortis viroid CEVd in vegetatively-propagated and seed-grown plants.

Immediate online access to all issues from Subscription will auto renew annually. References Bar-Joseph, M. Baranwal Authors Rudra P. Singh View author publications. You can also search for this author in PubMed Google Scholar. View author publications. Rights and permissions Reprints and Permissions. About this article Cite this article Singh, R.

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Citrus exocortis

Metrics details. The distributions and infection rates of two viroids have not been investigated since the two diseases were presented decades ago. The genetic diversities and evolutionary relationships of two viroids also remain unclear in the mix citrus planted region. Multiplex RT-PCR was used to detect the two viroids for the first time in seven main cultivars of citrus. Sequence alignment and phylogenetic analysis were performed using the ClustalW and MEGA6 neighbor-joining with p-distance model , respectively. Results of the multiplex quantitative analysis suggested that uneven distributions of both viroids with twig bark as the most appropriate material for studies involving viroid sampling such as quarantine inspection.

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Viroids are naked RNAs that do not code for any known protein and yet are able to infect plants causing severe diseases. Because of their RNA nature, many studies have focused on the involvement of viroids in RNA-mediated gene silencing as being their pathogenesis mechanism. Here, the alterations caused by the Citrus exocortis viroid CEVd on the tomato translation machinery were studied as a new aspect of viroid pathogenesis. The presence of viroids in the ribosomal fractions of infected tomato plants was detected. More precisely, CEVd and its derived viroid small RNAs were found to co-sediment with tomato ribosomes in vivo , and to provoke changes in the global polysome profiles, particularly in the 40S ribosomal subunit accumulation. Additionally, the viroid caused alterations in ribosome biogenesis in the infected tomato plants, affecting the 18S rRNA maturation process.

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We'd like to understand how you use our websites in order to improve them. Register your interest. Surveys of Impatiens and Verbena species in local nurseries in Fredericton, Canada and Verbena species in New Delhi, India showed widespread infection of Citrus exocortis viroid CEVd in vegetatively-propagated and seed-grown plants. To determine viroid seed transmission, samples of eight varieties of Impatiens and 11 varieties of Verbena were obtained from four commercial sources.

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