Correspondence to :. One of the limiting major side effects of cisplatin is nephrotoxicity. Tribulus terrestris TT has been used as an synthetic or herbal protective agents for kidney disorders. The present study amid to investigate the Tribulus terrestris Hydroalcoholic extract effect on cisplatin-induced apoptosis in mice kidney.

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Correspondence to :. One of the limiting major side effects of cisplatin is nephrotoxicity. Tribulus terrestris TT has been used as an synthetic or herbal protective agents for kidney disorders.

The present study amid to investigate the Tribulus terrestris Hydroalcoholic extract effect on cisplatin-induced apoptosis in mice kidney. Control group received saline, the first experimental group received cisplatin 5. The kidneys were removed after 4 days of injections, and TUNEL assay on mice's kidneys were performed. Weights of body and kidneys and apoptotic index were assessed. These parameters reached normal range after administration of fruit extracts of TT for 4 days.

The study demonstrates that extract of TT could have protective effect on cisplatin- induced apoptosis of kidney. This may be related to the presence of antioxidant components acting via a multitude of central and peripheral mechanisms.

Uno de los principales efectos secundarios limitantes del cisplatino es la nefrotoxicidad. Cisplatin cis-diamminedichloroplatinum II CP , a most effective chemotherapeutic agent, has been successfully used in treatment of a variety of solid tumors, including ovary, testis, bladder, head and neck, lung, cervix, and endometrium Baek et al.

It is an efficient platinum-derived alkylating agent that acts in unspecific phases of the cellular cycle against proliferating and resting cells Lirdi et al. CP nephrotoxicity pathological feature, the major adverse effect of CP in approximately one third of patients during chemotherapy in clinic, it has been the cause of renal cell damage, cell death and the loss of renal function or acute renal failure, especially in renal tubules Jiang et al.

Oxidative agents, principally involving reactive oxygen species ROS in renal tubular cells, are considered as the main mechanisms of nephrotoxicity of CP. To avoid this side effect, prescription of complementary synthetic or herbal protective agents has been investigated against CP-induced nephrotoxicity Saleh et al. Tribulus terrestris L grows with a wide distribution in tropical and moderate areas, including the US and Mexico, the Mediterranean region, and throughout Asia Phillips et al.

A fruit of traditional Chinese medicine Sugunavarman et al. Different parts of Tribulus terrestris L have been used traditionally in treating a variety of diseases including hypertension and coronary artery diseases, diabetes and hyperlipidemia, as well as fungal diseases Sharma et al. Tribulus terrestris L is also reported to have cooling, diuretic, tonic and aphrodisiac effects Sugunavarman et al.

Accordingly, it is of particular interest to discover the effects of Tribulus terrestris L on cisplatin-induced apoptosis in mice kidney. Collection and preparation of aqueous extract.

Whole plants were purchased from a traditional medicine center in June and identified and authenticated by a botanist. Then, fresh fruits of T. Extracting method was described previously Keshtmand et al.

Then, the tinctured grains were extracted by percolation method and extract concentrated in a vacuum and dried in the flat surface. The obtained extract weight, 6. After dissolving the extract in distilled water, extract was immediately administered interaperitoneally to mice.

Ten to fifteen minutes before use, CP was dissolved in saline in darkness. At one day of the experiment an intraperitoneal injection 5. Animal experiment and Groups. The animals were provided with standard diet pellets and water ad libitum. Experiments were started after one week of adaptation. Research protocols were approved by the local ethics committee. Mice were distributed at random into five groups each of six mice and individually put in metabolic cages and treated as follows: i control group C received normal saline 0.

Body weights of the mice were recorded initially and at the end of the experimental procedure day 5. Weights of the kidneys were also noted. Sections were dewaxed with xylene and rehydrated by standard methods and washed in deionized water. Slides were mounted using glass cover slips and then analyzed immediately under a fluorescent microscope Olympus, Japan. Apoptotic index AI was calculated by dividing the number of TUNEL positive cells to total number of the cells in randomly selected fields, and the result was multiplied by Lirdi et al.

Data analysis. After 4 days of injections CIS in a dose of 5. Renal tubular apoptosis has been suggested as a mechanism of cisplatin-induced acute kidney injury. The effect of toxic dose of cisplatin CIS 5. The effect of toxic dose of cisplatin 5. The present study demonstrated T. Treatment of mice with CP resulted in renal damage, showing a significant decline in the weight of animals treated with CP.

These data supported other studies that indicated reductions in body weight could be attributable to toxic side effect of chemotherapeutic drugs and it suggests that T. A single dose of CP caused apoptosis in kidney. Treatment of cisplatin-treated mice with T. Thus, together with other herbal extracts, T. The antioxidant activity of T. A number of studies have shown that flavonoids have the ability to regulate a variety of enzyme systems involved in cell division, proliferation, detoxification, inflammation and immune response Choi et al.

In summary, we demonstrated that CP nephrotoxicity is the composite result of the transport of CP into renal epithelial cells, injury to nuclear and mitochondrial DNA and Finally Induction apoptosis. In conclusion, our previous and present studies indicate that the antioxidant action of T.

Treatment of male mice with extract of T. An, Y. Amelioration of cisplatin-induced nephrotoxicity by pravastatin in mice. Arany, I. Cisplatin nephrotoxicity. Arjumand, W. Rutin attenuates cisplatin induced renal inflammation and apoptosis by reducing NFkB, TNF-a and caspase-3 expression in wistar rats. Food Chem. Ashrafi, F. A combination of vitamin C and losartan for cisplatin-induced nephrotoxicity in rats. Kidney Dis. Baek, S. Differential roles of hydrogen peroxide and hydroxyl radical in cisplatin-induced cell death in renal proximal tubular epithelial cells.

Choi, J. Flavones mitigate tumor necrosis factor-alpha-induced adhesion molecule upregulation in cultured human endothelial cells: role of nuclear factor-kappa B. Chu, S. Effect of saponin from Tribulus terrestris on hyperlipidemia. Zhong Yao Cai, 26 5 , Di Carlo, G. Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sci. Hadjzadeh, M. Effect of alcoholic extract of Nigella sativa on cisplatin-induced toxicity in rat.

Harborne, J. Advances in flavonoid research since Phytochemistry, 55 6 , Hollman, P. Dietary flavonoids: intake, health effects and bioavailability. Jiang, M. Cisplatin-induced apoptosis in pdeficient renal cells via the intrinsic mitochondrial pathway. Renal Physiol. Kang, K. Luteolin ameliorates cisplatin-induced acute kidney injury in mice by regulation of pdependent renal tubular apoptosis.

Kavitha, A. Role of Tribulus terrestris Linn. Zygophyllacea against mercuric chloride induced nephrotoxicity in mice, Mus musculus Linn. Kavitha, P. Hepatoprotective activity of Tribulus terrestris extract against acetaminophen-induced toxicity in a freshwater fish Oreochromis mossambicus. In Vitro Cell. Keshtmand, Z.

Lirdi, L. Amifostine protective effect on cisplatin-treated rat testis. Hoboken , 7 , Matsushima, H.



Important User Information: Remote access to EBSCO's databases is permitted to patrons of subscribing institutions accessing from remote locations for personal, non-commercial use. However, remote access to EBSCO's databases from non-subscribing institutions is not allowed if the purpose of the use is for commercial gain through cost reduction or avoidance for a non-subscribing institution. Source: Electronic Journal of Biomedicine. Jan-Apr, Issue 1, p Abstract: Cisplatin nephrotoxicity should always be considered as a potential cause of renal damage in acute renal failure installed in the context of its use. Among its mechanisms of renal functional impairment are: acute tubular necrosis, proximal tubular and loop of Henle dysfunctions, as well as the deterioration of various organelles, especially the mitochondria.


Tribulus terrestris Hydroalcoholic Extract Effect on Cisplatin-Induced Apoptosis in Mice Kidney.


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